eclipse ni-e confocal laser scanning microscope Search Results


non laser confocal microscopy apo tome confocal system  (Carl Zeiss)
90
Carl Zeiss non laser confocal microscopy apo tome confocal system
Non Laser Confocal Microscopy Apo Tome Confocal System, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vonfocal laser scanning microscopy  (Carl Zeiss)
90
Carl Zeiss vonfocal laser scanning microscopy
Vonfocal Laser Scanning Microscopy, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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510 spectrally resolved laser-scanning confocal microscope  (Carl Zeiss)
90
Carl Zeiss 510 spectrally resolved laser-scanning confocal microscope
510 Spectrally Resolved Laser Scanning Confocal Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a1r laser scanning confocal microscopy  (Nikon)
99
Nikon a1r laser scanning confocal microscopy
( A ) Representative live confocal <t>microscopy</t> of MAIT cells ( n = 2) migrating toward E. coli –pulsed PDAOs ( n = 5). Expanded and CellTrace Violet–labeled (CTV-) MAIT cells (blue) were cultured outside the Matrigel ECM domes, and confocal microscopy images were acquired 3 days later. E. coli embedded in Matrigel ECM alone served as a control for background MAIT cell migration into the Matrigel ECM domes. ( B ) In selected experiments ( n = 3), E. coli –pulsed appendix-derived organoids were stained using E-cadherin monoclonal antibody (mAb) (red) to examine their interactions with CTV-MAIT cells (blue). Representative live confocal microscopy images from two independent experiments are shown. ( C ) Expanded MAIT cells were cultured in MAIT cell medium without exogenous cytokines for 48 hours, labeled with CTV, then cultured outside the Matrigel ECM domes of E. coli –pulsed PDAO with or without IL-2 + IL-7, IL-12 + IL-18, or IL-1β + IL-23. Representative live confocal images from seven independent experiments acquired 2 days later show migrating CTV-MAIT cells (blue) infiltrating PDAO visualized by E-cadherin expression (red) and SYTO21-stained nuclei (green). ( D ) Following live confocal microcopy in (C), the cultures were fixed and stained intracellularly for Krt20 expression (yellow) to identify enterocytes. Normalized fluorescence intensities (Norm. fluor.) of CTV (MAIT cells) and Krt20 to SYTO21 [(C) and (D)] or E-cadherin (C) were shown. Confocal images were acquired with Nikon <t>A1R+</t> Laser scanning confocal microscope with 4×/0.2 [(A) and (B), left] and 10×/0.45 [(B), right, (C), and (D)] objectives. Imaging was controlled using the Nikon NIS-Elements software and processed with ImageJ 1.53a software. Scale bars, 100 μm.
A1r Laser Scanning Confocal Microscopy, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axiovert 200 equipped carv ii confocal imager  (Carl Zeiss)
90
Carl Zeiss axiovert 200 equipped carv ii confocal imager
( A ) Representative live confocal <t>microscopy</t> of MAIT cells ( n = 2) migrating toward E. coli –pulsed PDAOs ( n = 5). Expanded and CellTrace Violet–labeled (CTV-) MAIT cells (blue) were cultured outside the Matrigel ECM domes, and confocal microscopy images were acquired 3 days later. E. coli embedded in Matrigel ECM alone served as a control for background MAIT cell migration into the Matrigel ECM domes. ( B ) In selected experiments ( n = 3), E. coli –pulsed appendix-derived organoids were stained using E-cadherin monoclonal antibody (mAb) (red) to examine their interactions with CTV-MAIT cells (blue). Representative live confocal microscopy images from two independent experiments are shown. ( C ) Expanded MAIT cells were cultured in MAIT cell medium without exogenous cytokines for 48 hours, labeled with CTV, then cultured outside the Matrigel ECM domes of E. coli –pulsed PDAO with or without IL-2 + IL-7, IL-12 + IL-18, or IL-1β + IL-23. Representative live confocal images from seven independent experiments acquired 2 days later show migrating CTV-MAIT cells (blue) infiltrating PDAO visualized by E-cadherin expression (red) and SYTO21-stained nuclei (green). ( D ) Following live confocal microcopy in (C), the cultures were fixed and stained intracellularly for Krt20 expression (yellow) to identify enterocytes. Normalized fluorescence intensities (Norm. fluor.) of CTV (MAIT cells) and Krt20 to SYTO21 [(C) and (D)] or E-cadherin (C) were shown. Confocal images were acquired with Nikon <t>A1R+</t> Laser scanning confocal microscope with 4×/0.2 [(A) and (B), left] and 10×/0.45 [(B), right, (C), and (D)] objectives. Imaging was controlled using the Nikon NIS-Elements software and processed with ImageJ 1.53a software. Scale bars, 100 μm.
Axiovert 200 Equipped Carv Ii Confocal Imager, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5-live fast clsm  (Carl Zeiss)
90
Carl Zeiss 5-live fast clsm
( A ) Representative live confocal <t>microscopy</t> of MAIT cells ( n = 2) migrating toward E. coli –pulsed PDAOs ( n = 5). Expanded and CellTrace Violet–labeled (CTV-) MAIT cells (blue) were cultured outside the Matrigel ECM domes, and confocal microscopy images were acquired 3 days later. E. coli embedded in Matrigel ECM alone served as a control for background MAIT cell migration into the Matrigel ECM domes. ( B ) In selected experiments ( n = 3), E. coli –pulsed appendix-derived organoids were stained using E-cadherin monoclonal antibody (mAb) (red) to examine their interactions with CTV-MAIT cells (blue). Representative live confocal microscopy images from two independent experiments are shown. ( C ) Expanded MAIT cells were cultured in MAIT cell medium without exogenous cytokines for 48 hours, labeled with CTV, then cultured outside the Matrigel ECM domes of E. coli –pulsed PDAO with or without IL-2 + IL-7, IL-12 + IL-18, or IL-1β + IL-23. Representative live confocal images from seven independent experiments acquired 2 days later show migrating CTV-MAIT cells (blue) infiltrating PDAO visualized by E-cadherin expression (red) and SYTO21-stained nuclei (green). ( D ) Following live confocal microcopy in (C), the cultures were fixed and stained intracellularly for Krt20 expression (yellow) to identify enterocytes. Normalized fluorescence intensities (Norm. fluor.) of CTV (MAIT cells) and Krt20 to SYTO21 [(C) and (D)] or E-cadherin (C) were shown. Confocal images were acquired with Nikon <t>A1R+</t> Laser scanning confocal microscope with 4×/0.2 [(A) and (B), left] and 10×/0.45 [(B), right, (C), and (D)] objectives. Imaging was controlled using the Nikon NIS-Elements software and processed with ImageJ 1.53a software. Scale bars, 100 μm.
5 Live Fast Clsm, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluoviev 5.0 software  (Evident Corporation)
90
Evident Corporation fluoviev 5.0 software
( A ) Representative live confocal <t>microscopy</t> of MAIT cells ( n = 2) migrating toward E. coli –pulsed PDAOs ( n = 5). Expanded and CellTrace Violet–labeled (CTV-) MAIT cells (blue) were cultured outside the Matrigel ECM domes, and confocal microscopy images were acquired 3 days later. E. coli embedded in Matrigel ECM alone served as a control for background MAIT cell migration into the Matrigel ECM domes. ( B ) In selected experiments ( n = 3), E. coli –pulsed appendix-derived organoids were stained using E-cadherin monoclonal antibody (mAb) (red) to examine their interactions with CTV-MAIT cells (blue). Representative live confocal microscopy images from two independent experiments are shown. ( C ) Expanded MAIT cells were cultured in MAIT cell medium without exogenous cytokines for 48 hours, labeled with CTV, then cultured outside the Matrigel ECM domes of E. coli –pulsed PDAO with or without IL-2 + IL-7, IL-12 + IL-18, or IL-1β + IL-23. Representative live confocal images from seven independent experiments acquired 2 days later show migrating CTV-MAIT cells (blue) infiltrating PDAO visualized by E-cadherin expression (red) and SYTO21-stained nuclei (green). ( D ) Following live confocal microcopy in (C), the cultures were fixed and stained intracellularly for Krt20 expression (yellow) to identify enterocytes. Normalized fluorescence intensities (Norm. fluor.) of CTV (MAIT cells) and Krt20 to SYTO21 [(C) and (D)] or E-cadherin (C) were shown. Confocal images were acquired with Nikon <t>A1R+</t> Laser scanning confocal microscope with 4×/0.2 [(A) and (B), left] and 10×/0.45 [(B), right, (C), and (D)] objectives. Imaging was controlled using the Nikon NIS-Elements software and processed with ImageJ 1.53a software. Scale bars, 100 μm.
Fluoviev 5.0 Software, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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zen 2009 software  (Carl Zeiss)
90
Carl Zeiss zen 2009 software
( A ) Representative live confocal <t>microscopy</t> of MAIT cells ( n = 2) migrating toward E. coli –pulsed PDAOs ( n = 5). Expanded and CellTrace Violet–labeled (CTV-) MAIT cells (blue) were cultured outside the Matrigel ECM domes, and confocal microscopy images were acquired 3 days later. E. coli embedded in Matrigel ECM alone served as a control for background MAIT cell migration into the Matrigel ECM domes. ( B ) In selected experiments ( n = 3), E. coli –pulsed appendix-derived organoids were stained using E-cadherin monoclonal antibody (mAb) (red) to examine their interactions with CTV-MAIT cells (blue). Representative live confocal microscopy images from two independent experiments are shown. ( C ) Expanded MAIT cells were cultured in MAIT cell medium without exogenous cytokines for 48 hours, labeled with CTV, then cultured outside the Matrigel ECM domes of E. coli –pulsed PDAO with or without IL-2 + IL-7, IL-12 + IL-18, or IL-1β + IL-23. Representative live confocal images from seven independent experiments acquired 2 days later show migrating CTV-MAIT cells (blue) infiltrating PDAO visualized by E-cadherin expression (red) and SYTO21-stained nuclei (green). ( D ) Following live confocal microcopy in (C), the cultures were fixed and stained intracellularly for Krt20 expression (yellow) to identify enterocytes. Normalized fluorescence intensities (Norm. fluor.) of CTV (MAIT cells) and Krt20 to SYTO21 [(C) and (D)] or E-cadherin (C) were shown. Confocal images were acquired with Nikon <t>A1R+</t> Laser scanning confocal microscope with 4×/0.2 [(A) and (B), left] and 10×/0.45 [(B), right, (C), and (D)] objectives. Imaging was controlled using the Nikon NIS-Elements software and processed with ImageJ 1.53a software. Scale bars, 100 μm.
Zen 2009 Software, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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confocal laser scanning microscopy principles  (Carl Zeiss)
90
Carl Zeiss confocal laser scanning microscopy principles
( A ) Representative live confocal <t>microscopy</t> of MAIT cells ( n = 2) migrating toward E. coli –pulsed PDAOs ( n = 5). Expanded and CellTrace Violet–labeled (CTV-) MAIT cells (blue) were cultured outside the Matrigel ECM domes, and confocal microscopy images were acquired 3 days later. E. coli embedded in Matrigel ECM alone served as a control for background MAIT cell migration into the Matrigel ECM domes. ( B ) In selected experiments ( n = 3), E. coli –pulsed appendix-derived organoids were stained using E-cadherin monoclonal antibody (mAb) (red) to examine their interactions with CTV-MAIT cells (blue). Representative live confocal microscopy images from two independent experiments are shown. ( C ) Expanded MAIT cells were cultured in MAIT cell medium without exogenous cytokines for 48 hours, labeled with CTV, then cultured outside the Matrigel ECM domes of E. coli –pulsed PDAO with or without IL-2 + IL-7, IL-12 + IL-18, or IL-1β + IL-23. Representative live confocal images from seven independent experiments acquired 2 days later show migrating CTV-MAIT cells (blue) infiltrating PDAO visualized by E-cadherin expression (red) and SYTO21-stained nuclei (green). ( D ) Following live confocal microcopy in (C), the cultures were fixed and stained intracellularly for Krt20 expression (yellow) to identify enterocytes. Normalized fluorescence intensities (Norm. fluor.) of CTV (MAIT cells) and Krt20 to SYTO21 [(C) and (D)] or E-cadherin (C) were shown. Confocal images were acquired with Nikon <t>A1R+</t> Laser scanning confocal microscope with 4×/0.2 [(A) and (B), left] and 10×/0.45 [(B), right, (C), and (D)] objectives. Imaging was controlled using the Nikon NIS-Elements software and processed with ImageJ 1.53a software. Scale bars, 100 μm.
Confocal Laser Scanning Microscopy Principles, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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laser-scanning microscope 510 meta confocal microscope with a ×63 oil immersion lens  (Carl Zeiss)
90
Carl Zeiss laser-scanning microscope 510 meta confocal microscope with a ×63 oil immersion lens
( A ) Representative live confocal <t>microscopy</t> of MAIT cells ( n = 2) migrating toward E. coli –pulsed PDAOs ( n = 5). Expanded and CellTrace Violet–labeled (CTV-) MAIT cells (blue) were cultured outside the Matrigel ECM domes, and confocal microscopy images were acquired 3 days later. E. coli embedded in Matrigel ECM alone served as a control for background MAIT cell migration into the Matrigel ECM domes. ( B ) In selected experiments ( n = 3), E. coli –pulsed appendix-derived organoids were stained using E-cadherin monoclonal antibody (mAb) (red) to examine their interactions with CTV-MAIT cells (blue). Representative live confocal microscopy images from two independent experiments are shown. ( C ) Expanded MAIT cells were cultured in MAIT cell medium without exogenous cytokines for 48 hours, labeled with CTV, then cultured outside the Matrigel ECM domes of E. coli –pulsed PDAO with or without IL-2 + IL-7, IL-12 + IL-18, or IL-1β + IL-23. Representative live confocal images from seven independent experiments acquired 2 days later show migrating CTV-MAIT cells (blue) infiltrating PDAO visualized by E-cadherin expression (red) and SYTO21-stained nuclei (green). ( D ) Following live confocal microcopy in (C), the cultures were fixed and stained intracellularly for Krt20 expression (yellow) to identify enterocytes. Normalized fluorescence intensities (Norm. fluor.) of CTV (MAIT cells) and Krt20 to SYTO21 [(C) and (D)] or E-cadherin (C) were shown. Confocal images were acquired with Nikon <t>A1R+</t> Laser scanning confocal microscope with 4×/0.2 [(A) and (B), left] and 10×/0.45 [(B), right, (C), and (D)] objectives. Imaging was controlled using the Nikon NIS-Elements software and processed with ImageJ 1.53a software. Scale bars, 100 μm.
Laser Scanning Microscope 510 Meta Confocal Microscope With A ×63 Oil Immersion Lens, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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laser scanning confocal microscopy x63 planapo 1.4 aperture objective  (Carl Zeiss)
90
Carl Zeiss laser scanning confocal microscopy x63 planapo 1.4 aperture objective
( A ) Representative live confocal <t>microscopy</t> of MAIT cells ( n = 2) migrating toward E. coli –pulsed PDAOs ( n = 5). Expanded and CellTrace Violet–labeled (CTV-) MAIT cells (blue) were cultured outside the Matrigel ECM domes, and confocal microscopy images were acquired 3 days later. E. coli embedded in Matrigel ECM alone served as a control for background MAIT cell migration into the Matrigel ECM domes. ( B ) In selected experiments ( n = 3), E. coli –pulsed appendix-derived organoids were stained using E-cadherin monoclonal antibody (mAb) (red) to examine their interactions with CTV-MAIT cells (blue). Representative live confocal microscopy images from two independent experiments are shown. ( C ) Expanded MAIT cells were cultured in MAIT cell medium without exogenous cytokines for 48 hours, labeled with CTV, then cultured outside the Matrigel ECM domes of E. coli –pulsed PDAO with or without IL-2 + IL-7, IL-12 + IL-18, or IL-1β + IL-23. Representative live confocal images from seven independent experiments acquired 2 days later show migrating CTV-MAIT cells (blue) infiltrating PDAO visualized by E-cadherin expression (red) and SYTO21-stained nuclei (green). ( D ) Following live confocal microcopy in (C), the cultures were fixed and stained intracellularly for Krt20 expression (yellow) to identify enterocytes. Normalized fluorescence intensities (Norm. fluor.) of CTV (MAIT cells) and Krt20 to SYTO21 [(C) and (D)] or E-cadherin (C) were shown. Confocal images were acquired with Nikon <t>A1R+</t> Laser scanning confocal microscope with 4×/0.2 [(A) and (B), left] and 10×/0.45 [(B), right, (C), and (D)] objectives. Imaging was controlled using the Nikon NIS-Elements software and processed with ImageJ 1.53a software. Scale bars, 100 μm.
Laser Scanning Confocal Microscopy X63 Planapo 1.4 Aperture Objective, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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confocal laser scanning microscope zeiss imager z2  (Carl Zeiss)
90
Carl Zeiss confocal laser scanning microscope zeiss imager z2
( A ) Representative live confocal <t>microscopy</t> of MAIT cells ( n = 2) migrating toward E. coli –pulsed PDAOs ( n = 5). Expanded and CellTrace Violet–labeled (CTV-) MAIT cells (blue) were cultured outside the Matrigel ECM domes, and confocal microscopy images were acquired 3 days later. E. coli embedded in Matrigel ECM alone served as a control for background MAIT cell migration into the Matrigel ECM domes. ( B ) In selected experiments ( n = 3), E. coli –pulsed appendix-derived organoids were stained using E-cadherin monoclonal antibody (mAb) (red) to examine their interactions with CTV-MAIT cells (blue). Representative live confocal microscopy images from two independent experiments are shown. ( C ) Expanded MAIT cells were cultured in MAIT cell medium without exogenous cytokines for 48 hours, labeled with CTV, then cultured outside the Matrigel ECM domes of E. coli –pulsed PDAO with or without IL-2 + IL-7, IL-12 + IL-18, or IL-1β + IL-23. Representative live confocal images from seven independent experiments acquired 2 days later show migrating CTV-MAIT cells (blue) infiltrating PDAO visualized by E-cadherin expression (red) and SYTO21-stained nuclei (green). ( D ) Following live confocal microcopy in (C), the cultures were fixed and stained intracellularly for Krt20 expression (yellow) to identify enterocytes. Normalized fluorescence intensities (Norm. fluor.) of CTV (MAIT cells) and Krt20 to SYTO21 [(C) and (D)] or E-cadherin (C) were shown. Confocal images were acquired with Nikon <t>A1R+</t> Laser scanning confocal microscope with 4×/0.2 [(A) and (B), left] and 10×/0.45 [(B), right, (C), and (D)] objectives. Imaging was controlled using the Nikon NIS-Elements software and processed with ImageJ 1.53a software. Scale bars, 100 μm.
Confocal Laser Scanning Microscope Zeiss Imager Z2, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Representative live confocal microscopy of MAIT cells ( n = 2) migrating toward E. coli –pulsed PDAOs ( n = 5). Expanded and CellTrace Violet–labeled (CTV-) MAIT cells (blue) were cultured outside the Matrigel ECM domes, and confocal microscopy images were acquired 3 days later. E. coli embedded in Matrigel ECM alone served as a control for background MAIT cell migration into the Matrigel ECM domes. ( B ) In selected experiments ( n = 3), E. coli –pulsed appendix-derived organoids were stained using E-cadherin monoclonal antibody (mAb) (red) to examine their interactions with CTV-MAIT cells (blue). Representative live confocal microscopy images from two independent experiments are shown. ( C ) Expanded MAIT cells were cultured in MAIT cell medium without exogenous cytokines for 48 hours, labeled with CTV, then cultured outside the Matrigel ECM domes of E. coli –pulsed PDAO with or without IL-2 + IL-7, IL-12 + IL-18, or IL-1β + IL-23. Representative live confocal images from seven independent experiments acquired 2 days later show migrating CTV-MAIT cells (blue) infiltrating PDAO visualized by E-cadherin expression (red) and SYTO21-stained nuclei (green). ( D ) Following live confocal microcopy in (C), the cultures were fixed and stained intracellularly for Krt20 expression (yellow) to identify enterocytes. Normalized fluorescence intensities (Norm. fluor.) of CTV (MAIT cells) and Krt20 to SYTO21 [(C) and (D)] or E-cadherin (C) were shown. Confocal images were acquired with Nikon A1R+ Laser scanning confocal microscope with 4×/0.2 [(A) and (B), left] and 10×/0.45 [(B), right, (C), and (D)] objectives. Imaging was controlled using the Nikon NIS-Elements software and processed with ImageJ 1.53a software. Scale bars, 100 μm.

Journal: Science Advances

Article Title: MAIT cell activation and recruitment in inflammation and tissue damage in acute appendicitis

doi: 10.1126/sciadv.adn6331

Figure Lengend Snippet: ( A ) Representative live confocal microscopy of MAIT cells ( n = 2) migrating toward E. coli –pulsed PDAOs ( n = 5). Expanded and CellTrace Violet–labeled (CTV-) MAIT cells (blue) were cultured outside the Matrigel ECM domes, and confocal microscopy images were acquired 3 days later. E. coli embedded in Matrigel ECM alone served as a control for background MAIT cell migration into the Matrigel ECM domes. ( B ) In selected experiments ( n = 3), E. coli –pulsed appendix-derived organoids were stained using E-cadherin monoclonal antibody (mAb) (red) to examine their interactions with CTV-MAIT cells (blue). Representative live confocal microscopy images from two independent experiments are shown. ( C ) Expanded MAIT cells were cultured in MAIT cell medium without exogenous cytokines for 48 hours, labeled with CTV, then cultured outside the Matrigel ECM domes of E. coli –pulsed PDAO with or without IL-2 + IL-7, IL-12 + IL-18, or IL-1β + IL-23. Representative live confocal images from seven independent experiments acquired 2 days later show migrating CTV-MAIT cells (blue) infiltrating PDAO visualized by E-cadherin expression (red) and SYTO21-stained nuclei (green). ( D ) Following live confocal microcopy in (C), the cultures were fixed and stained intracellularly for Krt20 expression (yellow) to identify enterocytes. Normalized fluorescence intensities (Norm. fluor.) of CTV (MAIT cells) and Krt20 to SYTO21 [(C) and (D)] or E-cadherin (C) were shown. Confocal images were acquired with Nikon A1R+ Laser scanning confocal microscope with 4×/0.2 [(A) and (B), left] and 10×/0.45 [(B), right, (C), and (D)] objectives. Imaging was controlled using the Nikon NIS-Elements software and processed with ImageJ 1.53a software. Scale bars, 100 μm.

Article Snippet: The images were acquired with a Nikon A1R+ laser scanning confocal microscopy equipped with 4×/0.2, 10×/0.45, and 20×/0.45 objectives (Nikon).

Techniques: Confocal Microscopy, Labeling, Cell Culture, Migration, Derivative Assay, Staining, Expressing, Fluorescence, Microscopy, Imaging, Software